allergens allergy biosynthesis biotechnology congresses escherichiacoli geneexpression geneticengineering geneticintervention genetics geneticvectors hypersensitivity immediate immunology immunotherapy industrialmicrobiology laboratorymanuals pichia pichiapastoris proteinconformation proteinengineering proteins qw25c913p2007 recombinantmicroorganisms ribonucleases saccharomycescerevisiae schizosaccharomycespombe synthesis wd300n53171996 yeastfungi yeasts