1996g-763 agargel analysis biochemicalmarkers biologicalmarkers cardiovascularsystem chromatography diseases electrophoresis gelelectrophoresis geneexpression heart immunoblotting isoelectricfocusing isolation mass matrix-assistedlaserdesorption-ionization methodology methods molecularaspects polyacrylamide proteins proteomics purification qu25b691p1996 qu25z91999 research sequenceanalysis spectrometry two-dimensional two-dimensionalelectrophoresis